recombinant human cxcl16 protein  (PeproTech)

Journal: Clinical Cancer Research

Article Title: Redirecting B7-H3.CAR T Cells to Chemokines Expressed in Osteosarcoma Enhances Homing and Antitumor Activity in Preclinical Models

doi: 10.1158/1078-0432.CCR-23-3298

Figure Lengend Snippet: RNA-seq and multiplex analyses identify chemokines produced by primary OS samples. A, Expression of 28 chemokines by RNA-seq (FPKM shown; n = 85 OS samples); screen cutoff shown at 1 FPKM. B, Expression of 28 chemokines by protein secretion ( n = 8 patient samples); screen cutoff shown at 3 × 10 3 integrated pixel density. Green bars in A and B denote ligands deemed positive in both RNA and protein screens; error bars indicate SEM. C, Chemokine receptors (ligands in parentheses) CXCR2 (IL8), CXCR3 (CXCL10), CXCR6 (CXCL16), CCR7 (CCL21), and CCR8 (CCL18) as expressed endogenously and detected by flow cytometry on activated T cells ( n = 5 healthy donors; error bars indicate SEM). D, ELISA demonstrating detection of IL8 and CXCL16 in patient transport media specimens ( n = 6 patient tumor samples, 1 normal bone sample; error bars indicate SEM).

Article Snippet: At time point 0, the transwell was placed into a fresh V-shaped lower-chamber plate with 100 μL of RPMI supplemented with 1% FBS and 0, 10, or 30 ng/mL of recombinant human IL8 (R&D Systems) or recombinant human CXCL16 (R&D Systems).

Techniques: RNA Sequencing Assay, Multiplex Assay, Produced, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4 + macrophage infiltration

doi: 10.1038/s41467-024-51096-0

Figure Lengend Snippet: A Representative flow plots show the frequency of the CXCR6 + CD8 + GZMB + T cells (left) and quantification of the proportions of CXCR6 + CD8 + GZMB + T cells (right) in groups with different PF4 + Macrophage and CD8 + T-cell proportions. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. B Representative flow plots show the frequency of the CXCR6 + CD8 + PDCD1 + T cells (left) and quantification of the proportions of CXCR6 + CD8 + PDCD1 + T cells (right) in groups with different PF4 + Macrophage and CD8 + T-cell proportions. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. C , D Representative flow plots show frequency of the CXCR6 + CD8 + GZMB + T cells ( C ) and quantification of the proportions of CXCR6 + CD8 + GZMB + T cells ( D ) in groups with different concentrations of CXCL16 recombinant protein. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. E , F Representative flow plots show the frequency of the CXCR6 + CD8 + PDCD1 + T cells ( E ) and quantification of the proportions of CXCR6 + CD8 + PDCD1 + T cells ( F ) in groups with different concentrations of CXCL16 recombinant protein. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. G Representative images of 4NQO-induced HNSCC (upper) and corresponding HE staining(lower) in groups with different concentrations of CXCL16 recombinant protein. Scale bar, 1 mm (upper), 100 μm (lower). H – K Representative images of KI67 ( H ) and Caspase-3 ( J ) IHC staining and quantitation of the percentage of KI67 + ( I ) and Caspase-3 + ( K ) cells in groups with different concentrations of CXCL16 recombinant protein. Scale bar, 50 μm. Data are shown as mean ± SD (n = 8 mice). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. Source data and exact p values are provided as a Source Data file.

Article Snippet: In the co-culture system, the Moc1 cells were treated with different proportions of CD8 + T cells and PF4 + Macrophages, or with different concentrations of CXCL16 recombinant protein (HY-P700266, MCE).

Techniques: Comparison, Recombinant, Staining, Immunohistochemistry, Quantitation Assay

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: ELK3 depletion increases CXCL16 expression in TNBC cells.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: Expressing

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: The chemotactic activity of CXCL16 recruits NK cells to target TNBC cells.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: Activity Assay

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: The ELK3-CXCL16 axis determines the response of NK cells in TNBC cells.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques:

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: CXCL16 promotes NK cell chemotaxis and cytotoxicity in vivo .

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: Chemotaxis Assay, In Vivo

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: Negative correlation between ELK3 and CXCL16 in human breast cancer.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: